Bursa Uludag University, Faculty of Sciences and Letters, Biology Department
Genetic Toxicology
Laboratory
Comet Assay
Comet assay (also known as Single Cell Gel Electrophoresis - SCGE) is a fast and reliable technique which is used to detect DNA strand breaks
γH2AX Test
This test is used to evaluate double stranded DNA breaks via immunofluorescent visualisation of the phosphorylated H2AX accumulated at the sites of DSB
Micronucleus Test
The micronucleus test detects structural (clastogenic) or numerical (aneugenic) chromosomal aberrations in interphase cells, following chemical treatment.
Clonogenic Assay
Clonogenic or colony formation assay is a technique based on the ability of a single cell to grow into a colony used to determine the effectiveness of specific agents on the survival and proliferation of cells
XTT Test
In this viabillty assay, yellow tetrazolium salt XTT is cleaved to a soluble orange formazan dye, which can be measured by absorbance at 490 (or 450) nm in a microplate reader.
Intracellular ROS Test
This test is based on evaluation of changes in intracellular reactive oxygen species (ROS) levels using the fluorescebt microlplate reader
Annexin V-Fitc Apoptozis Test
Staining of cells using this staining technique allows to determination of apoptotic/nkecrotic cell rations uder fluorescent microscope.
Chromosomal Aberration Test
This test is used to aveluate numerical and structural chromosome aberratipons in metahpase plates following exposure to any toxic agent.